rapamycin signaling pathway  (Musashi Engineering Inc)

Journal: JAAD Case Reports

Article Title: Treatment of extramammary Paget’s disease with sirolimus: A case series

doi: 10.1016/j.jdcr.2025.10.021

Figure Lengend Snippet: Clinical images of patient 1 at baseline (A) , 8 wks on sirolimus twice daily (B) , and 16 wks on sirolimus twice daily (C) .

Article Snippet: While the pathogenesis of EMPD is not completely understood, the Musashi-1- mammalian target of rapamycin signaling pathway is thought to play a role in disease development.

Techniques:

Journal: JAAD Case Reports

Article Title: Treatment of extramammary Paget’s disease with sirolimus: A case series

doi: 10.1016/j.jdcr.2025.10.021

Figure Lengend Snippet: Clinical images of patient 3 at baseline (A) , 4 wks on sirolimus twice daily (B) , and 8 wks on sirolimus twice daily (C) .

Article Snippet: While the pathogenesis of EMPD is not completely understood, the Musashi-1- mammalian target of rapamycin signaling pathway is thought to play a role in disease development.

Techniques:

Journal: Molecular and cellular endocrinology

Article Title: N-acetylcysteine influence on PI3K/Akt/mTOR and sphingolipid pathways in rats with MASLD induced by HFD: a promising new therapeutic purpose.

doi: 10.1016/j.mce.2025.112545

Figure Lengend Snippet: Scheme 1. The visualization of N-acetylcysteine docking sites in selected proteins from PI3K/Akt/mTOR pathway. IRS1, insulin receptor substrate (7PPL); PTEN, phosphate and tensin homolog (7JTX); Akt, protein kinase B (4EQM); GSK3α/β, glycogen synthase kinase 3α/β (1I09); BAD, Bcl-2 associated agonist of cell death (7Q16); mTOR, mechanistic target of rapamycin (6SB0); P70 S6 kinase, P70 ribosomal protein S6 kinase (3A62); S6RP, S6 ribosomal protein (2WNT).

Article Snippet: The content of the phosphorylated form of proteins from phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt)/mechanistic target of rapamycin (mTOR) insulin signaling pathway as follows insulin receptor substrate (pIRS1(Ser636/Ser639)), phosphate and tensin homolog (pPTEN(Ser380)), pAkt(Ser473), glycogen synthase kinase 3α/β (pGSK3α/β(Ser21/Ser9)), Bcl-2 associated agonist of cell death (pBAD (Ser136)), pmTOR(Ser2448), P70 ribosomal protein S6 kinase (pP70 S6 kinase(Thr389)), S6 ribosomal protein (pS6RP(Ser235/Ser236)) was measured by the use of Bio-Plex Pro Cell Signaling Assays (Bio-Rad, Hercules, CA, USA).

Techniques:

Journal: bioRxiv

Article Title: Cholangiocytes contribute to hepatocyte regeneration after partial liver injury during growth spurt in zebrafish

doi: 10.1101/2025.01.09.629100

Figure Lengend Snippet: (A) UMAP visualization showing the integration of uninjured zebrafish larval and adult liver cells. (B) Unsupervised clustering of cell types presents in larval and adult datasets. The dashed area indicates cholangiocytes present in the two datasets. (C) Heatmap of mTORC1 signaling pathway-related gene expression in larval and adult cholangiocytes (top). Fold changes and p-values from differential gene expression (DGE) analysis displayed in dot plot (bottom). (D) Immunostaining for pS6 in uninjured larval and adult zebrafish liver sections. Scale bars: 10 μm. (E) Quantification of pS6 intensity in cholangiocytes from livers of uninjured larval and adult zebrafish. (F) Schematic of the experimental approach for inhibiting the mTORC1 signalling pathway in Tg(fabp10a:CellCousin) zebrafish larvae. (G) Confocal images of the liver from 3 dpi Tg(fabp10a:CellCousin) larvae treated with DMSO or 10 μM Rapamycin. Scale bars: 100 μm. (H) Quantification of normalized mTagBFP2+ de novo hepatocytes following treatment with Rapamycin treatment (n=17) compared to DMSO controls (n=17) (Mann-Whitney test).

Article Snippet: Inhibition of mTORC1 signaling pathway was performed using Rapamycin (MedChemExpress, HY-10219).

Techniques: Expressing, Immunostaining, MANN-WHITNEY

Journal: International Journal of Molecular Sciences

Article Title: The Comparative Oncology of Canine Malignant Melanoma in Targeted Therapy: A Systematic Review of In Vitro Experiments and Animal Model Reports

doi: 10.3390/ijms251910387

Figure Lengend Snippet: Summary of small molecule signaling inhibitors’ effectiveness in canine oral malignant melanoma.

Article Snippet: Rapamycin: Target cell signaling pathways mTOR , 12, 23, and 50 , 1. Decreases in phosphorylated mTOR expression and phosphorylated p70S6K expression. 2. Dose-dependent decrease in surviving tumor cell fraction. Cell line 12: At 0.1 nM rapamycin, the surviving fraction was between 0.28 and 0.38. At 1 nM, it dropped to 0.1, and at 100 nM, it was 0.1. Cell line 23: At 0.1 nM, the surviving fraction was similar to cell. , [ ] .

Techniques: Protein-Protein interactions, Expressing, In Vivo, In Vitro, Produced, Activity Assay, Binding Assay, Mutagenesis, Muscles, Biomarker Discovery, Phospho-proteomics, Inhibition, Migration, Blocking Assay